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Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium
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نویسنده
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maruyama r. ,choudhury s. ,kowalczyk a. ,bessarabova m. ,beresford-smith b. ,conway t. ,kaspi a. ,wu z. ,nikolskaya t. ,merino v.f. ,lo p.-k. ,liu x.s. ,nikolsky y. ,sukumar s. ,haviv i. ,polyak k.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 4
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چکیده
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Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type-specific features. understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles,which have been challenging in primary tissue samples due to limited numbers of cells available. here we describe the application of high-throughput sequencing technology for profiling histone and dna methylation,as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. we observed significant differences in histone h3 lysine 27 tri-methylation (h3k27me3) enrichment and dna methylation of genes expressed in a cell type-specific manner,suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. the technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. © 2011 maruyama et al.
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آدرس
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departments of medical oncology,dana-farber cancer institute,boston,ma,united states,department of medicine,brigham and women's hospital,boston,ma,united states,department of medicine,harvard medical school,boston,ma, United States, departments of medical oncology,dana-farber cancer institute,boston,ma,united states,department of medicine,brigham and women's hospital,boston,ma,united states,department of medicine,harvard medical school,boston,ma, United States, nicta victoria research laboratory,the university of melbourne,melbourne,vic,australia,department of electrical and electronic engineering,the university of melbourne,melbourne,vic, Australia, thomson reuters,healthcare and science,encinitas,ca, United States, nicta victoria research laboratory,the university of melbourne,melbourne,vic,australia,department of electrical and electronic engineering,the university of melbourne,melbourne,vic, Australia, department of electrical and electronic engineering,the university of melbourne,melbourne,vic,australia,department of computer science and software engineering,the university of melbourne,melbourne,vic, Australia, bioinformatics and system integration,baker idi heart and diabetes institute,melbourne,vic, Australia, department of biostatistics and computational biology,dana-farber cancer institute,boston,ma,united states,harvard school of public health,boston,ma, United States, thomson reuters,healthcare and science,encinitas,ca, United States, department of oncology,johns hopkins university school of medicine,baltimore,md, United States, department of oncology,johns hopkins university school of medicine,baltimore,md, United States, department of biostatistics and computational biology,dana-farber cancer institute,boston,ma,united states,harvard school of public health,boston,ma, United States, thomson reuters,healthcare and science,encinitas,ca, United States, department of oncology,johns hopkins university school of medicine,baltimore,md, United States, bioinformatics and system integration,baker idi heart and diabetes institute,melbourne,vic,australia,department of biochemistry,the university of melbourne,melbourne,vic,australia,metastasis research lab,peter maccallum cancer centre,melbourne,vic, Australia, departments of medical oncology,dana-farber cancer institute,boston,ma,united states,department of medicine,brigham and women's hospital,boston,ma,united states,department of medicine,harvard medical school,boston,ma,united states,harvard stem cell institute,cambridge,ma, United States
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Authors
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