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Assembly of drosophila centromeric chromatin proteins during mitosis
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نویسنده
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mellone b.g. ,grive k.j. ,shteyn v. ,bowers s.r. ,oderberg i. ,karpen g.h.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 5
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چکیده
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Semi-conservative segregation of nucleosomes to sister chromatids during dna replication creates gaps that must be filled by new nucleosome assembly. we analyzed the cell-cycle timing of centromeric chromatin assembly in drosophila,which contains the h3 variant cid (cenp-a in humans),as well as cenp-c and cal1,which are required for cid localization. pulse-chase experiments show that cid and cenp-c levels decrease by 50% at each cell division,as predicted for semi-conservative segregation and inheritance,whereas cal1 displays higher turnover. quench-chase-pulse experiments demonstrate that there is a significant lag between replication and replenishment of centromeric chromatin. surprisingly,new cid is recruited to centromeres in metaphase,by a mechanism that does not require an intact mitotic spindle,but does require proteasome activity. interestingly,new cal1 is recruited to centromeres before cid in prophase. furthermore,cal1,but not cenp-c,is found in complex with pre-nucleosomal cid. finally,cenp-c displays yet a different pattern of incorporation,during both interphase and mitosis. the unusual timing of cid recruitment and unique dynamics of cal1 identify a distinct centromere assembly pathway in drosophila and suggest that cal1 is a key regulator of centromere propagation. © 2011 mellone et al.
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آدرس
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department of molecular and cell biology,university of connecticut,storrs,ct, United States, department of molecular and cell biology,university of connecticut,storrs,ct,united states,department of molecular biology,cell biology,and biochemistry,brown university,providence,ri, United States, department of genome dynamics,lawrence berkeley national laboratory,berkeley,ca,united states,department of molecular and cell biology,university of california berkeley,berkeley,ca,united states,department of cell biology,yale university school of medicine,new haven,ct, United States, department of molecular and cell biology,university of connecticut,storrs,ct, United States, department of genome dynamics,lawrence berkeley national laboratory,berkeley,ca,united states,department of molecular and cell biology,university of california berkeley,berkeley,ca,united states,department of biology,massachusetts institute of technology,cambridge,ma, United States, department of genome dynamics,lawrence berkeley national laboratory,berkeley,ca,united states,department of molecular and cell biology,university of california berkeley,berkeley,ca, United States
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Authors
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