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A role for phosphatidic acid in the formation of supersized Lipid droplets
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نویسنده
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fei w. ,shui g. ,zhang y. ,krahmer n. ,ferguson c. ,kapterian t.s. ,lin r.c. ,dawes i.w. ,brown a.j. ,li p. ,huang x. ,parton r.g. ,wenk m.r. ,walther t.c. ,yang h.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 7
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چکیده
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Lipid droplets (lds) are important cellular organelles that govern the storage and turnover of lipids. little is known about how the size of lds is controlled,although lds of diverse sizes have been observed in different tissues and under different (patho)physiological conditions. recent studies have indicated that the size of lds may influence adipogenesis,the rate of lipolysis and the oxidation of fatty acids. here,a genome-wide screen identifies ten yeast mutants producing supersized lds that are up to 50 times the volume of those in wild-type cells. the mutated genes include: fld1,which encodes a homologue of mammalian seipin; five genes (cds1,ino2,ino4,cho2,and opi3) that are known to regulate phospholipid metabolism; two genes (ckb1 and ckb2) encoding subunits of the casein kinase 2; and two genes (mrps35 and rtc2) of unknown function. biochemical and genetic analyses reveal that a common feature of these mutants is an increase in the level of cellular phosphatidic acid (pa). results from in vivo and in vitro analyses indicate that pa may facilitate the coalescence of contacting lds,resulting in the formation of supersized lds. in summary,our results provide important insights into how the size of lds is determined and identify novel gene products that regulate phospholipid metabolism. © 2011 fei et al.
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آدرس
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school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, department of biochemistry,national university of singapore,singapore,singapore,department of biological sciences,national university of singapore,singapore, Singapore, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, department of cell biology,yale university school of medicine,new haven,ct, United States, institute for molecular bioscience,centre for microscopy and microanalysis,university of queensland,brisbane, Australia, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia, department of biological sciences and biotechnology,tsinghua university,beijing, China, laboratory of molecular and developmental biology,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, institute for molecular bioscience,centre for microscopy and microanalysis,university of queensland,brisbane, Australia, department of biochemistry,national university of singapore,singapore,singapore,department of biological sciences,national university of singapore,singapore, Singapore, department of cell biology,yale university school of medicine,new haven,ct, United States, school of biotechnology and biomolecular sciences,university of new south wales,sydney, Australia
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Authors
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