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Comparative and functional genomics of Rhodococcus opacus PD630 for Biofuels development
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نویسنده
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holder j.w. ,ulrich j.c. ,debono a.c. ,godfrey p.a. ,desjardins c.a. ,zucker j. ,zeng q. ,leach a.l.b. ,ghiviriga i. ,dancel c. ,abeel t. ,gevers d. ,kodira c.d. ,desany b. ,affourtit j.p. ,birren b.w. ,sinskey a.j.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 9
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چکیده
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The actinomycetales bacteria rhodococcus opacus pd630 and rhodococcus jostii rha1 bioconvert a diverse range of organic substrates through lipid biosynthesis into large quantities of energy-rich triacylglycerols (tags). to describe the genetic basis of the rhodococcus oleaginous metabolism,we sequenced and performed comparative analysis of the 9.27 mb r. opacus pd630 genome. metabolic-reconstruction assigned 2017 enzymatic reactions to the 8632 r. opacus pd630 genes we identified. of these,261 genes were implicated in the r. opacus pd630 tags cycle by metabolic reconstruction and gene family analysis. rhodococcus synthesizes uncommon straight-chain odd-carbon fatty acids in high abundance and stores them as tags. we have identified these to be pentadecanoic,heptadecanoic,and cis-heptadecenoic acids. to identify bioconversion pathways,we screened r. opacus pd630,r. jostii rha1,ralstonia eutropha h16,and c. glutamicum 13032 for growth on 190 compounds. the results of the catabolic screen,phylogenetic analysis of the tags cycle enzymes,and metabolic product characterizations were integrated into a working model of prokaryotic oleaginy. © 2011 holder et al.
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آدرس
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department of biology,massachusetts institute of technology,cambridge,ma,united states,the broad institute,cambridge,ma, United States, department of biology,massachusetts institute of technology,cambridge,ma, United States, department of biology,massachusetts institute of technology,cambridge,ma, United States, the broad institute,cambridge,ma, United States, the broad institute,cambridge,ma, United States, department of biology,massachusetts institute of technology,cambridge,ma,united states,the broad institute,cambridge,ma, United States, the broad institute,cambridge,ma, United States, department of biology,massachusetts institute of technology,cambridge,ma, United States, department of chemistry,university of florida,gainesville,fl, United States, department of chemistry,university of florida,gainesville,fl, United States, the broad institute,cambridge,ma, United States, the broad institute,cambridge,ma, United States, 454 life sciences,branford,ct, United States, 454 life sciences,branford,ct, United States, 454 life sciences,branford,ct, United States, the broad institute,cambridge,ma, United States, department of biology,massachusetts institute of technology,cambridge,ma,united states,division of health sciences and technology,massachusetts institute of technology,cambridge,ma,united states,engineering systems division,massachusetts institute of technology,cambridge,ma, United States
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Authors
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