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Transcriptome-wide binding sites for components of the Saccharomyces cerevisiae non-poly(A) termination pathway: Nrd1,Nab3,and Sen1
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نویسنده
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creamer t.j. ,darby m.m. ,jamonnak n. ,schaughency p. ,hao h. ,wheelan s.j. ,corden j.l.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 10
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چکیده
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Rna polymerase ii synthesizes a diverse set of transcripts including both protein-coding and non-coding rnas. one major difference between these two classes of transcripts is the mechanism of termination. messenger rna transcripts terminate downstream of the coding region in a process that is coupled to cleavage and polyadenylation reactions. non-coding transcripts like saccharomyces cerevisiae snornas terminate in a process that requires the rna-binding proteins nrd1,nab3,and sen1. we report here the transcriptome-wide distribution of these termination factors. these data sets derived from in vivo protein-rna cross-linking provide high-resolution definition of non-poly(a) terminators,identify novel genes regulated by attenuation of nascent transcripts close to the promoter,and demonstrate the widespread occurrence of nrd1-bound 3′ antisense transcripts on genes that are poorly expressed. in addition,we show that sen1 does not cross-link efficiently to many expected non-coding rnas but does cross-link to the 3′ end of most pre-mrna transcripts,suggesting an extensive role in mrna 3′ end formation and/or termination. © 2011 creamer et al.
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آدرس
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department of molecular biology and genetics,the johns hopkins university school of medicine,baltimore,md, United States, department of molecular biology and genetics,the johns hopkins university school of medicine,baltimore,md, United States, department of molecular biology and genetics,the johns hopkins university school of medicine,baltimore,md, United States, department of molecular biology and genetics,the johns hopkins university school of medicine,baltimore,md, United States, high throughput biology center,the johns hopkins university school of medicine,baltimore,md, United States, department of oncology,the johns hopkins university school of medicine,baltimore,md,united states,department of biostatistics,bloomberg school of public health,the johns hopkins university,baltimore,md, United States, department of molecular biology and genetics,the johns hopkins university school of medicine,baltimore,md, United States
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Authors
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