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A systems biology approach reveals the role of a novel methyltransferase in response to chemical stress and lipid homeostasis
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نویسنده
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lissina e. ,young b. ,urbanus m.l. ,guan x.l. ,lowenson j. ,hoon s. ,baryshnikova a. ,riezman i. ,michaut m. ,riezman h. ,cowen l.e. ,wenk m.r. ,clarke s.g. ,giaever g. ,nislow c.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 10
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چکیده
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Using small molecule probes to understand gene function is an attractive approach that allows functional characterization of genes that are dispensable in standard laboratory conditions and provides insight into the mode of action of these compounds. using chemogenomic assays we previously identified yeast crg1,an uncharacterized sam-dependent methyltransferase,as a novel interactor of the protein phosphatase inhibitor cantharidin. in this study we used a combinatorial approach that exploits contemporary high-throughput techniques available in saccharomyces cerevisiae combined with rigorous biological follow-up to characterize the interaction of crg1 with cantharidin. biochemical analysis of this enzyme followed by a systematic analysis of the interactome and lipidome of crg1 mutants revealed that crg1,a stress-responsive sam-dependent methyltransferase,methylates cantharidin in vitro. chemogenomic assays uncovered that lipid-related processes are essential for cantharidin resistance in cells sensitized by deletion of the crg1 gene. lipidome-wide analysis of mutants further showed that cantharidin induces alterations in glycerophospholipid and sphingolipid abundance in a crg1-dependent manner. we propose that crg1 is a small molecule methyltransferase important for maintaining lipid homeostasis in response to drug perturbation. this approach demonstrates the value of combining chemical genomics with other systems-based methods for characterizing proteins and elucidating previously unknown mechanisms of action of small molecule inhibitors. © 2011 lissina et al.
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آدرس
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department of molecular genetics,university of toronto,toronto,canada,terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto, Canada, department of chemistry and biochemistry,the molecular biology institute,university of california los angeles,los angeles,ca, United States, terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto,canada,banting and best department of medical research,university of toronto,toronto, Canada, department of biological sciences,yong loo lin school of medicine,national university of singapore,singapore,singapore,department of biochemistry,university of geneva,geneva, Switzerland, department of chemistry and biochemistry,the molecular biology institute,university of california los angeles,los angeles,ca, United States, molecular engineering lab,agency for science,technology,and research,singapore, Singapore, department of molecular genetics,university of toronto,toronto,canada,terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto, Canada, department of biochemistry,university of geneva,geneva, Switzerland, terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto, Canada, department of biochemistry,university of geneva,geneva, Switzerland, department of molecular genetics,university of toronto,toronto, Canada, department of biological sciences,yong loo lin school of medicine,national university of singapore,singapore, Singapore, department of chemistry and biochemistry,the molecular biology institute,university of california los angeles,los angeles,ca, United States, department of molecular genetics,university of toronto,toronto,canada,terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto,canada,department of pharmacy and pharmaceutical sciences,university of toronto,toronto, Canada, department of molecular genetics,university of toronto,toronto,canada,terrence donnelly centre for cellular and biomolecular research,university of toronto,toronto,canada,banting and best department of medical research,university of toronto,toronto, Canada
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Authors
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