|
|
|
|
Feed-forward microprocessing and splicing activities at a microRNA-containing intron
|
|
|
|
|
|
|
|
نویسنده
|
janas m.m. ,khaled m. ,schubert s. ,bernstein j.g. ,golan d. ,veguilla r.a. ,fisher d.e. ,shomron n. ,levy c. ,novina c.d.
|
|
منبع
|
plos genetics - 2011 - دوره : 7 - شماره : 10
|
|
چکیده
|
The majority of mammalian microrna (mirna) genes reside within introns of protein-encoding and non-coding genes,yet the mechanisms coordinating primary transcript processing into both mature mirna and spliced mrna are poorly understood. analysis of melanoma invasion suppressor mir-211 expressed from intron 6 of melastatin revealed that microprocessing of mir-211 promotes splicing of the exon 6-exon 7 junction of melastatin by a mechanism requiring the rnase iii activity of drosha. additionally,mutations in the 5′ splice site (5′ss),but not in the 3′ss,branch point,or polypyrimidine tract of intron 6 reduced mir-211 biogenesis and drosha recruitment to intron 6,indicating that 5′ss recognition by the spliceosome promotes microprocessing of mir-211. globally,knockdown of u1 splicing factors reduced intronic mirna expression. our data demonstrate novel mutually-cooperative microprocessing and splicing activities at an intronic mirna locus and suggest that the initiation of spliceosome assembly may promote microprocessing of intronic mirnas. © 2011 janas et al.
|
|
|
|
|
آدرس
|
department of cancer immunology and aids,dana-farber cancer institute,department of microbiology and immunobiology,harvard medical school,boston,ma,united states,broad institute of harvard and mit,cambridge,ma, United States, department of cancer immunology and aids,dana-farber cancer institute,department of microbiology and immunobiology,harvard medical school,boston,ma,united states,broad institute of harvard and mit,cambridge,ma,united states,cutaneous biology research center,harvard medical school,charlestown,ma, United States, department of cancer immunology and aids,dana-farber cancer institute,department of microbiology and immunobiology,harvard medical school,boston,ma,united states,broad institute of harvard and mit,cambridge,ma,united states,cenix bioscience,dresden, Germany, mit media lab,massachusetts institute of technology,cambridge,ma, United States, department of cell and developmental biology,sackler faculty of medicine,tel aviv university,tel aviv, Israel, cutaneous biology research center,harvard medical school,charlestown,ma, United States, cutaneous biology research center,harvard medical school,charlestown,ma, United States, department of cell and developmental biology,sackler faculty of medicine,tel aviv university,tel aviv, Israel, department of human genetics and biochemistry,sackler faculty of medicine,tel aviv university,tel aviv, Israel, department of cancer immunology and aids,dana-farber cancer institute,department of microbiology and immunobiology,harvard medical school,boston,ma,united states,broad institute of harvard and mit,cambridge,ma, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|