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Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways
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نویسنده
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babu m. ,díaz-mejía j.j. ,vlasblom j. ,gagarinova a. ,phanse s. ,graham c. ,yousif f. ,ding h. ,xiong x. ,nazarians-armavil a. ,alamgir m. ,ali m. ,pogoutse o. ,pe'er a. ,arnold r. ,michaut m. ,parkinson j. ,golshani a. ,whitfield c. ,wodak s.j. ,moreno-hagelsieb g. ,greenblatt j.f. ,emili a.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 11
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چکیده
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As the interface between a microbe and its environment,the bacterial cell envelope has broad biological and clinical significance. while numerous biosynthesis genes and pathways have been identified and studied in isolation,how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. to this end,we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. the differential patterns of genetic interactions detected among >235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. these networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target. © 2011 babu et al.
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آدرس
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banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto,canada,department of biology,wilfrid laurier university,waterloo, Canada, hospital for sick children,toronto,canada,department of biochemistry,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto,canada,department of molecular genetics,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, hospital for sick children,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, department of biology,ottawa institute of systems biology,carleton university,ottawa, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, department of microbiology and molecular genetics,institute for medical research-israel-canada,the hebrew university of jerusalem,jerusalem, Israel, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto, Canada, hospital for sick children,toronto,canada,department of biochemistry,university of toronto,toronto,canada,department of molecular genetics,university of toronto,toronto, Canada, department of biology,ottawa institute of systems biology,carleton university,ottawa, Canada, department of molecular and cellular biology,university of guelph,guelph, Canada, hospital for sick children,toronto,canada,department of biochemistry,university of toronto,toronto,canada,department of molecular genetics,university of toronto,toronto, Canada, department of biology,wilfrid laurier university,waterloo, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto,canada,department of molecular genetics,university of toronto,toronto, Canada, banting and best department of medical research,donnelly centre,university of toronto,toronto,canada,department of molecular genetics,university of toronto,toronto, Canada
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Authors
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