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Occurrence and multilocus genotyping of Giardia intestinalis assemblage C and D in farmed raccoon dogs,Nyctereutes procyonoides,in China
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نویسنده
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zhang x.-x. ,zheng w.-b. ,ma j.-g. ,yao q.-x. ,zou y. ,bubu c.-j. ,zhao q. ,zhu x.-q.
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منبع
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parasites and vectors - 2016 - دوره : 9 - شماره : 1
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چکیده
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Background: giardia intestinalis,the only causative agent of human giardiasis,can infect a wide range of animals. as no information concerning the prevalence and genotyping of g. intestinalis in raccoon dogs in china is available,examination of 305 faecal samples from raccoon dogs in jilin province (n = 110),heilongjiang province (n = 40),liaoning province (n = 72),hebei province (n = 54) and shandong province (n = 29) was conducted to estimate the prevalence of g. intestinalis in raccoon dogs in northern china and identify their genotypes using a genetic approach. findings: of 305 faecal samples from farmed raccoon dogs,22 (7.21 %) were detected g. intestinalis-positive by nested pcr amplification of the triosephosphate isomerase (tpi) gene. the prevalence of g. intestinalis was strongly related to the region and season of sampling. all 22 samples were analysed at the tpi,the glutamate dehydrogenase (gdh) and the beta giardin (bg) gene loci,showing 13,3,2 subtypes,respectively. the results also demonstrated that two raccoon dogs harboured mixed infections of assemblage c and assemblage d (or mixed c/d),whereas only assemblage c was detected in the remaining 20 samples. moreover,five new multilocus genotypes,named as mlgs c1-c5,were observed in the assemblage c in the present study. conclusions: this is the first report of g. intestinalis infection in raccoon dogs in china. dna sequence analysis of the tpi,gdh and bg gene indicated that 13,3,2 subtypes were found at these loci,respectively. furthermore,this is also the first report of five new multilocus genotypes (mlgs c1-c5) in farmed raccoon dogs,which provides baseline data for further studies of the distribution of g. duodenalis in different hosts. © 2016 the author(s).
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کلیدواژه
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China; Genotyping; Giardia intestinalis; Prevalence; Raccoon dogs
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آدرس
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state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,jiangsu co-innov. ctr. for the prev. and contr. of important animal infectious diseases and zoonoses,yangzhou university,college of veterinary medicine,yangzhou,jiangsu province,225009, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, college of animal science and technology,jilin agricultural university,changchun,jilin province,130118, China, state key laboratory of veterinary etiological biology,key laboratory of veterinary parasitology of gansu province,lanzhou veterinary research institute,chinese academy of agricultural sciences,lanzhou,gansu province,730046,china,college of animal science and technology,jilin agricultural university,changchun,jilin province,130118,china,jiangsu co-innov. ctr. for the prev. and contr. of important animal infectious diseases and zoonoses,yangzhou university,college of veterinary medicine,yangzhou,jiangsu province,225009, China
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Authors
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