Use of MALDI-TOF MS and culturomics to identify mosquitoes and their midgut microbiota

Background: mosquitoes transmit a wide range of human parasitic and viral diseases. in recent years,new techniques such as maldi-tof ms have been developed to identify mosquitoes at the species level,which is key for entomological surveys. additionally,there is increasing interest in the mosquito microbiota and its role in vector capacity. methods: the culturomics approach previously used in our laboratory to study human gut microbiota was applied to evaluate the midgut bacterial diversity of anopheles gambiae (wild and laboratory strains),aedes albopictus (wild and laboratory strains) and culex quinquefasciatus (wild strains) in order to determine the influence of the environmental status on the midgut microbiota of the mosquitoes. results: mosquitoes collected in the field were accurately identified by maldi-tof ms analysis of their legs. adult mosquito midgut microbiota was composed of four phyla,including proteobacteria,bacteroidetes,actinobacteria and firmicutes. the majority of the bacteria detected in the microbiota of mosquitoes were gram-negative and belong to the phylum proteobacteria. maldi-tof ms identified for the first time a new bacterial species from an. gambiae midgut microbiota. conclusion: in this study,the culturomics approach was found to be a reliable technique for exploring the diversity of the mosquito microbiota. maldi-tof ms was confirmed as a promising technique to identify mosquitoes collected in the field. culturomics allowed the isolation of a new bacterial species not previously associated with mosquito vectors. the environment plays a role in the bacterial diversity of the microbiota,which could enable the development of new control strategies for mosquito-borne disease. © 2016 the author(s).