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Evaluation of protective efficacy induced by different heterologous prime-boost strategies encoding triosephosphate isomerase against Schistosoma japonicum in mice
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نویسنده
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dai y. ,zhao s. ,tang j. ,xing y. ,qu g. ,dai j. ,jin x. ,wang x.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1 - صفحه:111
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چکیده
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Background: in china,schistosomiasis japonica is a predominant zoonotic disease,and animal reservoir hosts in the environment largely sustain infections. the development of transmission-blocking veterinary vaccines is urgently needed for the prevention and efficient control of schistosomiasis. heterologous prime-boost strategy is more effective than traditional vaccination and homologous prime-boost strategies against multiple pathogens infection. in the present study,to further improve protective efficacy,we immunized mice with three types of heterologous prime-boost combinations based on our previously constructed vaccines that encode triosphate isomerase of schistosoma japonicum,tested the specific immune responses,and evaluated the protective efficacy through challenge infection in mice.methods: dna vaccine (pcdna3.1-sjtpi.opt),adenoviral vectored vaccine (radv-sjtpi.opt),and recombinant protein vaccine (rsjtpi) were prepared and three types of heterologous prime-boost combinations,including dna i.m. priming-radv i.m. boosting,radv i.m. priming-radv s.c. boosting,and radv i.m. priming-rsjtpi boosting strategies,were carried out. the specific immune responses and protective efficacies were evaluated in balb/c mice results: results show that different immune profiles and various levels of protective efficacy were elicited by using different heterologous prime-boost combinations. a synergistic effect was observed using the dna i.m. priming-radv i.m. boosting strategy; however,its protective efficacy was similar to that of radv i.m. immunization. conversely,an antagonistic effect was generated by using the rad i.m. priming-s.c. boosting strategy. however,the strategy,with radv i.m. priming- rsjtpi s.c. boosting,generated the most optimal protective efficacy and worm or egg reduction rate reaching up to 70% in a mouse model.conclusions: a suitable immunization strategy,radv i.m. priming-rsjtpi boosting strategy,was developed,which elicits a high level of protective efficacy against schistosoma japonicum infection in mice.
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کلیدواژه
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Heterologous prime-boost strategy; Protective efficacy; Schistosoma japonicum; Triosphosphate isomerase; Vaccination
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آدرس
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key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china. jipddy@hotmail.com,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China, key laboratory of national health and family planning commission on parasitic disease control and prevention,jiangsu provincial key laboratory on parasite and vector control technology,jiangsu institute of parasitic diseases,wuxi,jiangsu province,214064,people's republic of china,public health research center,jiangnan university,wuxi,jiangsu province,214122, China
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