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Morphological and molecular characteristics of Malayfilaria sofiani Uni,Mat Udin and Takaoka n. g.,n. sp. (Nematoda: Filarioidea) from the common treeshrew Tupaia glis Diard and Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia
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نویسنده
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uni s. ,mat udin a.s. ,agatsuma t. ,saijuntha w. ,junker k. ,ramli r. ,omar h. ,lim y.a.-l. ,sivanandam s. ,lefoulon e. ,martin c. ,belabut d.m. ,kasim s. ,abdullah halim m.r. ,zainuri n.a. ,bhassu s. ,fukuda m. ,matsubayashi m. ,harada m. ,low v.l. ,chen c.d. ,suganuma n. ,hashim r. ,takaoka h. ,azirun m.s.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1
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چکیده
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Background: the filarial nematodes wuchereria bancrofti (cobbold,1877),brugia malayi (brug,1927) and b. timori partono,purnomo,dennis,atmosoedjono,oemijati and cross,1977 cause lymphatic diseases in humans in the tropics,while b. pahangi (buckley and edeson,1956) infects carnivores and causes zoonotic diseases in humans in malaysia. wuchereria bancrofti,w. kalimantani palmieri,pulnomo,dennis and marwoto,1980 and six out of ten brugia spp. have been described from australia,southeast asia,sri lanka and india. however,the origin and evolution of the species in the wuchereria-brugia clade remain unclear. while investigating the diversity of filarial parasites in malaysia,we discovered an undescribed species in the common treeshrew tupaia glis diard and duvaucel (mammalia: scandentia). methods: we examined 81 common treeshrews from 14 areas in nine states and the federal territory of peninsular malaysia for filarial parasites. once any filariae that were found had been isolated,we examined their morphological characteristics and determined the partial sequences of their mitochondrial cytochrome c oxidase subunit 1 (cox1) and 12s rrna genes. polymerase chain reaction (pcr) products of the internal transcribed spacer 1 (its1) region were then cloned into the pgem-t vector,and the recombinant plasmids were used as templates for sequencing. results: malayfilaria sofiani uni,mat udin and takaoka,n. g.,n. sp. is described based on the morphological characteristics of adults and microfilariae found in common treeshrews from jeram pasu,kelantan,malaysia. the kimura 2-parameter distance between the cox1 gene sequences of the new species and w. bancrofti was 11.8%. based on the three gene sequences,the new species forms a monophyletic clade with w. bancrofti and brugia spp. the adult parasites were found in tissues surrounding the lymph nodes of the neck of common treeshrews. conclusions: the newly described species appears most closely related to wuchereria spp. and brugia spp.,but differs from these in several morphological characteristics. molecular analyses based on the cox1 and 12s rrna genes and the its1 region indicated that this species differs from both w. bancrofti and brugia spp. at the genus level. we thus propose a new genus,malayfilaria,along with the new species m. sofiani. © 2017 the author(s).
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کلیدواژه
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Filarial nematodes; Lymphatic filariosis; Molecular identification; Onchocercidae; Scandentia; Southeast Asia
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آدرس
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institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603,malaysia,department of parasitology,graduate school of medicine,osaka city university,osaka,545-8585, Japan, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, department of environmental medicine,kochi medical school,kochi university,nankoku,783-8505, Japan, department of environmental medicine,kochi medical school,kochi university,nankoku,783-8505,japan,walai rukhavej botanical research institute,mahasarakham university,mahasarakham,44150, Thailand, arc-onderstepoort veterinary institute,private bag x05,onderstepoort,0110, South Africa, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, department of parasitology,faculty of medicine,university of malaya,kuala lumpur,50603, Malaysia, department of parasitology,faculty of medicine,university of malaya,kuala lumpur,50603, Malaysia, umr7245,mcam,muséum national d’histoire naturelle,paris,75005, France, umr7245,mcam,muséum national d’histoire naturelle,paris,75005, France, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, research promotion institute,oita university,oita,879-5593, Japan, department of international prevention of epidemics,division of veterinary science,graduate school of life and environmental sciences,osaka prefecture university,osaka,598-8531, Japan, laboratory animal center,graduate school of medicine,osaka city university,osaka,545-8585, Japan, tropical infectious diseases research and education centre,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, department of environmental medicine,kochi medical school,kochi university,nankoku,783-8505, Japan, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia, institute of biological sciences,faculty of science,university of malaya,kuala lumpur,50603, Malaysia
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Authors
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