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Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring
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نویسنده
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da costa c.f. ,dos passos r.a. ,lima j.b.p. ,roque r.a. ,de souza sampaio v. ,campolina t.b. ,secundino n.f.c. ,pimenta p.f.p.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1
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چکیده
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Background: transovarial transmission of dengue virus in aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. however,there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. the present study evaluated transovarial transmission of dengue virus in aedes aegypti populations as a xenomonitoring strategy in municipalities of the amazonas state. results: aedes sp. eggs (13.164) were collected,with 30% viability of third- and fourth-instar larvae. transovarial transmission of denv was detected in all municipalities. the transovarial infection rate (tor) in the municipalities was 46% of the denv positive samples. the minimum infection rate (mir) was 17.7 in the state,varying from 11.4 to 24.1 per 1,000 larvae tested in the respective municipalities. four denv serotypes were identified,with denv i and iv being present in all municipalities investigated. the number of reported dengue fever cases varied during this period. conclusions: our results suggest that transovarial transmission may be an important mechanism for the maintenance and spreading of the disease in amazonas municipalities. using qrt-pcr,it was possible to identify the four denv serotypes in larval samples. the methodology used in the present study proved suitable as a denv xenomonitoring model in immature mosquitoes,contributing to the development of systems for early detection of viral circulation and predictive models for the occurrence of outbreaks and epidemics of this disease. trial registration: caae34025414200005015 . © 2017 the author(s).
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کلیدواژه
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Aedes aegypti; Control; Dengue; Monitoring; Ovitraps; Transovarial transmission; Xenosurveillance
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آدرس
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department of environmental surveillance,health surveillance foundation of amazonas state fvs-am,av. torquato tapajós,6132,colônia santo antônio,manaus,amazonas,zip 69.093-018,brazil,universidade nilton lins,programa de pró reitoria de pesquisa e pós-graduação,unicenter,laboratório de entomologia aplicada,office 160,av. professor nilton lins 3259,parque das laranjeiras,manaus,amazonas,zip: 69 058-030, Brazil, department of environmental surveillance,health surveillance foundation of amazonas state fvs-am,av. torquato tapajós,6132,colônia santo antônio,manaus,amazonas,zip 69.093-018,brazil,laboratory of physiology and control of arthropod vectors,oswaldo cruz institute,fiocruz,rio de janeiro, Brazil, laboratory of physiology and control of arthropod vectors,oswaldo cruz institute,fiocruz,rio de janeiro, Brazil, malaria and dengue laboratory,national institute of amazonian research (inpa),av. andré araújo,2.936 petrópolis,manaus,amazonas,zip 69080-971, Brazil, department of environmental surveillance,health surveillance foundation of amazonas state fvs-am,av. torquato tapajós,6132,colônia santo antônio,manaus,amazonas,zip 69.093-018, Brazil, rené rachou research centre,oswaldo cruz foundation,laboratory of medical entomology,belo horizonte,minas gerais,zip 30190-002, Brazil, rené rachou research centre,oswaldo cruz foundation,laboratory of medical entomology,belo horizonte,minas gerais,zip 30190-002, Brazil, rené rachou research centre,oswaldo cruz foundation,laboratory of medical entomology,belo horizonte,minas gerais,zip 30190-002, Brazil
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Authors
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