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Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China
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نویسنده
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wu c. ,zhang w. ,ran b. ,fan h. ,wang h. ,guo b. ,zhou c. ,shao y. ,zhang w. ,giraudoux p. ,knapp j. ,wen h. ,kuang l. ,li j.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1
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چکیده
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Background: alveolar echinococcosis (ae) is a life-threatening human disease caused by echinococcus multilocularis transmitted between rodents and dogs/foxes in the northern hemisphere. the study aims to identify the genetic variation of the parasite in ae patients from china. methods: e. multilocularis isolates were collected from wild small mammals (n = 6) and ae patients (n = 56) from western china. genomic dna was extracted from different tissue samples including paraffin tissue blocks,ethanol fixed tissues and frozen tissues surgically removed. two mitochondrial gene fragments (526 bp for cob and 474 bp for nad2) of e. multilocularis were amplified and sequenced. results: the parasite fragment sequences of cob fragments from ae patients showed two haplotypes,and nad2 gene fragment sequences had four haplotypes. the gene sequences from microtus sp. were 100% identical to the sequences of some isolates from ae patients. these haplotypes were distributed in both qinghai and xinjiang provinces. alignment analysis with the sequences from the genbank databases showed five genotypes including three asian genotypes,one from europe and one from north america. conclusions: most ae patients harbored the asian genotype 1 which may be an indication of its relative frequency in the definitive hosts and the environment or of its pathogenicity to humans,which calls for further research. © 2017 the author(s).
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کلیدواژه
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Alveolar echinococcosis; cob; Echinococcus multilocularis; Genetic variation; Mitochondrial genes; nad2
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آدرس
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clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi,china,collage of veterinary medicine,xinjiang agriculture university urumqi,xinjiang,830000, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China, centre for clinic echinococcosis,first teaching hospital,xinjiang medical university,urumqi,xinjiang 830000, China, department of hepatopancreatobiliary surgery,affiliated hospital of qinghai university,251 xining road,xi-ning,qinghai 810000, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China, centre for clinic echinococcosis,first teaching hospital,xinjiang medical university,urumqi,xinjiang 830000, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China, chrono-environmentlaboratory,university of bourgogne franche-comté,umr cnrs 6249,besançon,25000,france,institut universitaire de france,paris,75015, France, chrono-environmentlaboratory,university of bourgogne franche-comté,umr cnrs 6249,besançon,25000,france,department of parasitology-mycology,university hospital of besançon,besançon,25000, France, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi,china,centre for clinic echinococcosis,first teaching hospital,xinjiang medical university,urumqi,xinjiang 830000, China, collage of veterinary medicine,xinjiang agriculture university urumqi,xinjiang,830000, China, clinic medicine research institute,first teaching hospital,xinjiang medical university,urumqi, China
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Authors
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