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The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10,B17 and Th17 cells
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نویسنده
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pan w. ,hao w.-t. ,shen y.-j. ,li x.-y. ,wang y.-j. ,sun f.-f. ,yin j.-h. ,zhang j. ,tang r.-x. ,cao j.-p. ,zheng k.-y.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1
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چکیده
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Background: excretory-secretory products (esps) released by helminths are well-known to regulate t cell responses in the host. however,their direct influence in the differentiation of naïve t cells,and especially b cells,remains largely unknown. this study investigated the effects of echinococcus granulosus protoscoleces esps (egpsc-esps) on the differentiation of il-10-producing b cells (b10),il-17a-producing b cells (b17) and th17 cells. methods: balb/c mice injected with egpsc were used to evaluate the in vivo profiles of b10,b17 and th17 cells. in vitro purified cd19+ b and naïve cd4+ t cells were cultured in the presence of native,heat-inactivated or periodate-treated egpsc-esps,and the differentiation of these cell subsets were compared. results: in contrast to the control group,infected mice showed higher frequencies of b10,b17 and th17 cells,and higher levels of il-10 and il-17a in the sera. interestingly,b17 cells were first identified to express cd19+cd1dhigh. in vitro,b cells cultured with native esps exhibited a higher percentage of b10 cells but lower percentage of b17 and th17 cells compared to the pbs group. moreover,the relative expression of il-10 and il-17a mrna were consistent with the altered frequencies. however,esps subjected to heat-inactivation or periodate treatment exhibited an inverse effect on the induction of these cell subsets. conclusions: our findings indicate that esps released by egpsc can directly regulate the differentiation of b10,b17 and th17 cells,which appear to be heat-labile and carbohydrate-dependent. © 2017 the author(s).
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کلیدواژه
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Echinococcus granulosus protoscoleces; Excretory-secretory products; IL-10-producing B cells; IL-17A-producing B cells; Inflammation
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آدرس
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jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province,china,national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province, China, national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province, China, national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province, China, national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province, China, national institute of parasitic diseases,chinese center for disease control and prevention,key laboratory of parasite and vector biology,ministry of health,shanghai, China, jiangsu key laboratory of immunity and metabolism,department of pathogenic biology and immunology,laboratory of infection and immunity,xuzhou medical university,xuzhou,jiangsu province, China
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