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γ-Carboxymuconolactone decarboxylase: A novel cell cycle-related basal body protein in the early branching eukaryote Trichomonas vaginalis
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نویسنده
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cheng w.-h. ,huang k.-y. ,huang p.-j. ,lee c.-c. ,yeh y.-m. ,ku f.-m. ,lin r. ,cheng m.-l. ,chiu c.-h. ,tang p.
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منبع
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parasites and vectors - 2017 - دوره : 10 - شماره : 1
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چکیده
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Background: γ-carboxymuconolactone decarboxylase (cmd) participates in the β-ketoadipate pathway,which catalyzes aromatic compounds to produce acetyl- or succinyl-coa,in prokaryotes and yeast. our previous study demonstrated that expression of a cmd homologue that contains two signatures (dualcmd) is negatively regulated by iron in trichomonas vaginalis. however,we were not able to identify the components of the β-ketoadipate pathway in the parasite's genome. these observations prompted us to investigate the biological functions of this novel cmd homologue in t. vaginalis. methods: the specific anti-tvcmd1 antibody was generated,and the expression of tvcmd1 in t. vaginalis cultured under iron-rich and iron-deficient were evaluated. phylogenetic,metabolomic and substrate induction (protocatechuate and benzoate) analysis were conducted to clarify the function of dualcmd in trichomonad cells. subcellular localization of tvcmd1 was observed by confocal microscopy. the cell cycle-related role of tvcmd1 was assessed by treating cells with g2/m inhibitor nocodazole. results: we confirmed that t. vaginalis is not able to catabolize the aromatic compounds benzoate and protocatechuate,which are known substrates of the β-ketoadipate pathway. using immunofluorescence microscopy,we found that tvcmd1 is spatially associated with the basal body,a part of the cytoskeletal organizing center in t. vaginalis. tvcmd1 accumulated upon treatment with the g2/m inhibitor nocodazole. additionally,tvcmd1 was expressed and transported to/from the basal body during cytokinesis,suggesting that tvcmd1 plays a role in cell division. conclusion: we demonstrated that tvcmd1 is unlikely to participate in the β-ketoadipate pathway and demonstrated that it is a novel basal body-localizing (associated) protein. this model sheds light on the importance of genes that are acquired laterally in the coevolution of ancient protists,which surprisingly functions in cell cycle regulation of t. vaginalis. © 2017 the author(s).
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کلیدواژه
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Basal body; Cell cycle; Iron deficiency; Lateral gene transfer; Trichomonas vaginalis; γ-carboxymuconolactone decarboxylase
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آدرس
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graduate institute of biomedical sciences,college of medicine,chang gung university,kweishan,taoyuan,taiwan,molecular regulation and bioinformatics laboratory,department of parasitology,college of medicine,chang gung university,kweishan,taoyuan, Taiwan, graduate institute of pathology and parasitology,national defense medical center,taipei, Taiwan, graduate institute of biomedical sciences,college of medicine,chang gung university,kweishan,taoyuan,taiwan,bioinformatics core laboratory,molecular medicine research center,chang gung university,kweishan,taoyuan,taiwan,department of biomedical sciences,college of medicine,chang gung university,kweishan,taoyuan, Taiwan, department and graduate,institute of computer science and information engineering,college of engineering,chang gung university,kweishan,taoyuan, Taiwan, bioinformatics core laboratory,molecular medicine research center,chang gung university,kweishan,taoyuan, Taiwan, molecular regulation and bioinformatics laboratory,department of parasitology,college of medicine,chang gung university,kweishan,taoyuan, Taiwan, molecular regulation and bioinformatics laboratory,department of parasitology,college of medicine,chang gung university,kweishan,taoyuan, Taiwan, department of biomedical sciences,college of medicine,chang gung university,kweishan,taoyuan, Taiwan, molecular infectious disease research center,chang gung memorial hospital,kweishan,taoyuan, Taiwan, graduate institute of biomedical sciences,college of medicine,chang gung university,kweishan,taoyuan,taiwan,molecular regulation and bioinformatics laboratory,department of parasitology,college of medicine,chang gung university,kweishan,taoyuan,taiwan,bioinformatics core laboratory,molecular medicine research center,chang gung university,kweishan,taoyuan,taiwan,molecular infectious disease research center,chang gung memorial hospital,kweishan,taoyuan, Taiwan
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Authors
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