Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province,China

Background: the protistan pathogens cryptosporidium and giardia can cause significant intestinal diseases in animals and humans. cattle,particularly calves,carrying these protists can be significant reservoirs for human infections and disease. however,little is known about the genetic make-up of cryptosporidium and giardia populations in cattle and other ruminants in some regions of china. results: in the present study,pcr-based tools were used to genetically characterise these protists in faecal samples from a total of 339 pre- and post-weaned calves from four distinct locations in hubei province using markers in the large (lsu) or small (ssu) subunits of nuclear ribosomal rna genes. cryptosporidium andersoni,c. bovis,c. ryanae and giardia duodenalis assemblage e were detected in 0.6%,10.9%,4.1% and 22.6% of calves,respectively. conclusions: this study is the first to report the prevalence of cryptosporidium and giardia in pre- and post-weaned calves in hubei province,and encourages large-scale molecular studies of animals and humans,in an effort to better understand the epidemiology of these enteric pathogens in china. © 2017 the author(s).