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Exploring features and function of Ss-riok-3,an enigmatic kinase gene from Strongyloides stercoralis
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نویسنده
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yuan w. ,liu y. ,lok j.b. ,stoltzfus j.d. ,gasser r.b. ,lei w. ,fang r. ,junlong zhao state key laboratory of agricultural microbiology ,hu m.
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منبع
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parasites and vectors - 2014 - دوره : 7 - شماره : 1
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چکیده
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Background: right open reading frame protein kinase 3 (riok-3) belongs to the atypical kinase family. unlike th other two members,riok-1 and riok-2,which are conserved from archaea to humans,riok-3 occurs only i multicellular organisms. studies on hela cells indicate that human riok-3 is a component of the 40s small ribosom subunit and supports cancer cell growth and survival. however,almost nothing is known about the function of riok-3 we explored the functional role of riok-3 encoding gene from strongyloides stercoralis,a parasitic nematode of human and dogs methods: to analyze the gene and promoter structure of ss-riok-3,race-pcr and genome-walker pcr were performe to isolate the full length cdna,gdna and promoter region of ss-riok-3. rna-seq was conducted to assess the transcrip abundance of ss-riok-3 in different stages of s. stercoralis. transgenesis was employed to determine the anatomi expression patterns of ss-riok-3 results: the riok-3 protein-encoding gene (designated ss-riok-3) of s. stercoralis was characterized. the full-lengt complementary and genomic dnas of the riok-3 encoding gene (riok-3) were isolated from this nematode. the cdn of ss-riok-3 is 1,757 bp in length,including a 23 bp 5'-utr,a 36 bp 3'-utr and a 1,698 bp coding region encoding protein of 565 amino acids (aa) containing a rio kinase domain. rna sequencing (rna-seq) analysis revealed tha ss-riok-3 is transcribed in all developmental stages of s. stercoralis assessed,with transcripts being particularly abundan in parasitic females. gene structure analysis revealed that ss-riok-3 contains no intron. the putative promoter contain conserved promoter elements,including four tata,two gata,one inverse gata and one inverse caat boxes. th promoter of ss-riok-3 drives gfp expression in the head neuron,intestine and body wall muscle of transgeni s. stercoralis larvae,and the tata boxes present in the 3'-utr of the gene immediately upstream of ss-riok-3 initiat transcription conclusions: the characterization of the riok-3 encoding gene from s. stercoralis provides a sound foundation fo investigating in detail its function in the development and reproduction of this important pathogen. © 2014 wang et al.
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کلیدواژه
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Parasitic nematode; riok-3; Strongyloides stercoralis; Transgenesis
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آدرس
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state key laboratory of agricultural microbiology,key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China, state key laboratory of agricultural microbiology,key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China, department of pathobiology,school of veterinary medicine,university of pennsylvania,3800 spruce street,philadelphia,pa 19104, United States, department of pathobiology,school of veterinary medicine,university of pennsylvania,3800 spruce street,philadelphia,pa 19104,united states,department of biology,hollins university,roanoke,vi 24020, United States, faculty of veterinary and agricultural sciences,university of melbourne,corner of flemington road and park drive,parkville,vi 3010, Australia, state key laboratory of agricultural microbiology,key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China, state key laboratory of agricultural microbiology,key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China, key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China, state key laboratory of agricultural microbiology,key laboratory of development of veterinary diagnostic products,huazhong agricultural university,1 shizishan street,wuhan430070, China
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Authors
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