>
Fa   |   Ar   |   En
   Identification of phlebotomine sand flies (Diptera: Psychodidae) by matrix-assisted laser desorption/ionization time of flight mass spectrometry  
   
نویسنده dvorak v. ,halada p. ,hlavackova k. ,dokianakis e. ,antoniou m. ,volf p.
منبع parasites and vectors - 2014 - دوره : 7 - شماره : 1
چکیده    Background: phlebotomine sand flies are incriminated in the transmission of several human and veterinary pathogens. to elucidate their role as vectors,proper species identification is crucial. since traditional morphological determination is based on minute and often dubious characteristics on their head and genitalia,which require certain expertise and may be damaged in the field-collected material,there is a demand for rapid,simple and cost-effective molecular approaches. methods. six laboratory-reared colonies of phlebotomine sand flies belonging to five species and four subgenera (phlebotomus,paraphlebotomus,larroussius,adlerius) were used to evaluate the discriminatory power of matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms). various storage conditions and treatments,including the homogenization in either distilled water or given concentrations of formic acid,were tested on samples of both sexes. results: specimens of all five analysed sand fly species produced informative,reproducible and species-specific protein spectra that enabled their conclusive species identification. the method also distinguished between two p. sergenti colonies originating from different geographical localities. protein profiles within a species were similar for specimens of both sexes. tested conditions of specimen storage and sample preparation give ground to a standard protocol that is generally applicable on analyzed sand fly specimens. conclusions: species identification of sand flies by maldi-tof ms is feasible and represents a novel promising tool to improve biological and epidemiological studies on these medically important insects. © 2014 dvorak et al.; licensee biomed central ltd.
کلیدواژه MALDI-TOF MS; Molecular taxonomy; Phlebotomus; Species identification
آدرس department of parasitology,faculty of science,charles university in prague,prague, Czech Republic, laboratory of molecular structure characterization,institute of microbiology,academy of sciences of the czech republic,prague, Czech Republic, department of parasitology,faculty of science,charles university in prague,prague, Czech Republic, laboratory of clinical bacteriology,parasitology,zoonoses and geographical medicine,faculty of medicine,university of crete,crete, Greece, laboratory of clinical bacteriology,parasitology,zoonoses and geographical medicine,faculty of medicine,university of crete,crete, Greece, department of parasitology,faculty of science,charles university in prague,prague, Czech Republic
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved