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Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China
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نویسنده
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cai y.-c. ,xu j.-f. ,steinmann p. ,chen s.-h. ,chu y.-h. ,tian l.-g. ,chen m.-x. ,li h. ,lu y. ,zhang l.-l. ,zhou y. ,chen j.-x.
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منبع
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parasites and vectors - 2014 - دوره : 7 - شماره : 1
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چکیده
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Background: schistosomiasis remains a serious public health problem in affected countries,and routine,highly sensitive and cost-effective diagnostic methods are lacking. we evaluated two immunodiagnostic techniques for the detection of schistosoma japonicum infections: circulating antibody and circulating antigen assays. methods. a total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in hubei province were screened by serum examination with an indirect hemagglutination assay (iha). the positive individuals (titer ≥20 in iha) were reconfirmed by stool examination with the kato-katz method (three slides from a single stool specimen). samples of good serum quality and a volume above 0.5 ml were selected for further testing with two immunodiagnostic antibody (ddia and elisa) and two antigen (elisa) assays. results: the average antibody positive rate in the five villages was 12.7%,while the average parasitological prevalence was 1.50%; 25 of the 28 egg-positive samples were also circulating antigen-positive. significant differences were observed between the prevalence according to the kato-katz method and all three immunodiagnostic antibody assays (p-value <0.0001). similar differences were observed between the kato-katz method and the two immunodiagnostic antigen assays (p-value <0.0001) and between the antigen and antibody assays (p-value <0.0001). conclusion: both circulating antibody and circulating antigen assays had acceptable performance characteristics. immunodiagnostic techniques to detect circulating antigens have potential to be deployed for schistosomiasis japonica screening in the endemic areas. © 2014 cai et al.; licensee biomed central ltd.
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کلیدواژه
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China; Circulating antibody; Circulating antigen; Schistosoma japonicum
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آدرس
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national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, school of medicine,hubei university for nationalities,enshi,hubei 445000, China, department of epidemiology and public health,swiss tropical and public health institute,4051 basel,switzerland,university of basel,4051 basel, Switzerland, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China, national institute of parasitic diseases,chinese center for disease control and prevention,shanghai 200025,china,key laboratory of parasite and vector biology,ministry of health,shanghai 200025,china,who collaborating centre for malaria,schistosomiasis and filariasis,shanghai 200025, China
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Authors
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