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Genetic characterization of Toxoplasma gondii from cats in Yunnan Province,Southwestern China
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نویسنده
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tian y.-m. ,huang s.-y. ,miao q. ,jiang h.-h. ,yang j.-f. ,su c. ,zhu x.-q. ,zou f.-c.
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منبع
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parasites and vectors - 2014 - دوره : 7 - شماره : 1
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چکیده
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Background: cats are the definitive hosts of toxoplasma gondii. the distribution of genetic diversity of t. gondii in cats is of importance to understand the transmission of this parasite. the objective of this study was to genetically characterize t. gondii isolates from cats in yunnan province,southwestern china. methods. genomic dna was extracted from 5-10 g cat tissue samples (brain,tongue,heart,and liver). using multilocous polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technology,we determined genetic diversity of t. gondii isolates from cats in yunnan province. result: in total,175 stray cats were tested for t. gondii dna,respectively,44 (25.14%) of which were found to be positive for the t. gondii b1 gene by pcr amplification. the positive dna samples were typed at 11 genetic markers,including 10 nuclear markers,namely,sag1,5′-3′sag2,alternative sag2,sag3,gra6,l358,pk1,btub,c22-8,c29-2 and an apicoplast locus apico. of these,16 isolates from cats were genotyped with data for more than 9 loci,revealed 5 genotypes in total,of which 11 of 16 samples were identified as toxodb#9,two samples may belong to genotye #225,one was type ii,one was toxodb#3,and one was toxodb#20 (http://toxodb.org/toxo/). conclusions: the results of the present study indicated a wide distribution of t. gondii infection in cats in yunnan province,which may pose significant public health concerns. to our knowledge,the present study is the first report of t. gondii prevalence and genotypes in cats in southwestern china,and the first report of type ii t. gondii from cats in china. © 2014 tian et al.; licensee biomed central ltd.
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کلیدواژه
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Cats; Genetic characterization; Multilocus PCR-RFLP; Toxoplasma gondii; Yunnan
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آدرس
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college of animal science and technology,yunnan agricultural university,650201 kunming,yunnan province,china,state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province, China, state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province, China, college of animal science and technology,yunnan agricultural university,650201 kunming,yunnan province,china,state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province, China, state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province, China, college of animal science and technology,yunnan agricultural university,650201 kunming,yunnan province, China, state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province,china,department of microbiology,university of tennessee,37996 knoxville,tn, United States, college of animal science and technology,yunnan agricultural university,650201 kunming,yunnan province,china,state key laboratory of veterinary etiological biology,lanzhou veterinary research institute,chinese academy of agricultural sciences,730046 lanzhou,gansu province, China, college of animal science and technology,yunnan agricultural university,650201 kunming,yunnan province,china,school of life sciences,yunnan university,650091 kunming,yunnan province, China
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Authors
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