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Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon
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نویسنده
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santana r.a.g. ,magalhães l.k.c. ,magalhães l.k.c. ,prestes s.r. ,maciel m.g. ,da silva g.a.v. ,monteiro w.m. ,de brito f.r. ,de aguiar raposo câmara coelho l.i. ,barbosa-ferreira j.m. ,guerra j.a.o. ,silveira h. ,das graças vale barbosa m.
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منبع
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parasites and vectors - 2014 - دوره : 7 - شماره : 1
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چکیده
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Background: chagas disease in the amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (dtus) tci and tciv. these dtus are responsible for cases of acute disease associated with oral transmission. chronic disease cases have been detected through serological surveys. however,the mode of transmission could not be determined,or any association of chronic disease with a specific t. cruzi dtu's. the aim of this study was to characterize trypanosoma cruzi in patients with chronic chagas disease in the state of amazonas,brazil. methods. blood culture and xenodiagnosis were performed in 36 patients with positive serology for chagas disease who participated in a serological survey performed in urban and rural areas of manaus,amazonas. dna samples were extracted from the feces of triatomines used for xenodiagnosis,and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit ii (coii) were amplified by pcr and sequenced. results: blood culture and xenodiagnosis were negative in 100% of samples; however,molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis,t. cruzi was characterized as the dtu tci,and different haplotypes were identified within the same dtu. conclusion: the dtu tci,which is mainly associated with acute cases of chagas disease in the amazon region,is also responsible for chronic infection in patients from a region in the state of amazonas. © 2014 santana et al.; licensee biomed central ltd.
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آدرس
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university of the state of amazonas (universidade do estado do amazonas),manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus, Brazil, leônidas and maria deane institute,fiocruz of amazon,manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus,brazil,heitor vieira dourado tropical medicine foundation,manaus, Brazil, heitor vieira dourado tropical medicine foundation,manaus, Brazil, federal university of amazonas (universidade federal do amazonas),manaus, Brazil, university of the state of amazonas (universidade do estado do amazonas),manaus,brazil,francisca mendes hospital,manaus, Brazil, heitor vieira dourado tropical medicine foundation,manaus, Brazil, institute of hygiene and tropical medicine (instituto de higiene e medicina tropical),new university of lisbon (universidade nova de lisboa),lisbon, Portugal, university of the state of amazonas (universidade do estado do amazonas),manaus,brazil,heitor vieira dourado tropical medicine foundation,manaus, Brazil
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Authors
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r.a.g. ,l.k.c. ,l.k.c. ,s.r. ,m.g. ,g.a.v. ,w.m. ,f.r. ,l.i. ,j.m. ,j.a.o. ,h. ,m.
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