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Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil
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نویسنده
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lima costa c.r. ,freitas m.t.d.s. ,santiago figueirêdo c.a. ,aragão n.c. ,da silva l.g. ,marcondes c.b. ,dias r.v. ,leal-balbino t.c. ,souza m.b.r. ,ramalho-ortigão m. ,balbino v.d.q.
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منبع
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parasites and vectors - 2015 - دوره : 8 - شماره : 1
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چکیده
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Background: even one hundred years after being originally identified,aspects of the taxonomy of the sand fly lutzomyia longipalpis,the principal vector of leishmania infantum in the americas,remain unresolved for brazilian populations of this vector. the diversity of morphological,behavioral,biochemical,and ethological characters,as well as the genetic variability detected by molecular markers are indicative of the presence of a complex of species. methods: in this study,a 525 bp fragment of the period gene was used to evaluate sympatric populations of l. longipalpis. a combination of probabilistic methods such as maximum likelihood and genetic assignment approach to investigate sympatric species of l. longipalpis were applied in three populations of northeast brazil. results: fixed polymorphisms in geographically isolated populations of l. longipalpis from two localities in the state of ceará and one in the state of pernambuco,brazil,was identified in a 525 bp fragment of the gene period (per). our results suggest a direct relationship between the number of spots found in males' tergites and the genetic variation in cryptic species of l. longipalpis. the fragment used in this study revealed the nature of the ancestral morphotype 1s. conclusion: new polymorphisms were identified in the gene per which can be used as a genetic barcode to sympatric taxonomy of l. longipalpis. the per gene fragment confirmed the presence of two siblings species of l. longipalpis in sobral and showed that these same species are present in two other localities,representing an expansion within the l. longipalpis species complex with regards to the states of ceará and pernambuco. © 2015 lima costa et al.; licensee biomed central.
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کلیدواژه
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Fixed polymorphism; Lutzomyia longipalpis complex; Period gene
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آدرس
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jr.,departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, jr.,departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, departamento de microbiologia,imunologia e parasitologia,universidade federal de santa catarina,campus reitor joão david ferreira lima,florianópolis,santa catarina,88040-900, Brazil, centro de controle de zoonoses,rua finlândia s/n,parque silvana ii,sobral,ceara,62010-970, Brazil, departamento de microbiologia,centro de pesquisas aggeu magalhaes,cidade universitária,avenida professor moraes rego s/n,recife,pernambuco,50740-465, Brazil, departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil, department of entomology,kansas state university,w. waters hall 123,manhattan,ks 66506-400, United States, departamento de genética,universidade federal de pernambuco,cidade universitária,avenida da engenharia s/n,recife,pernambuco,50.740-600, Brazil
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Authors
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