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Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia
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نویسنده
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gebresilassie a. ,abbasi i. ,aklilu e. ,yared s. ,kirstein o.d. ,moncaz a. ,tekie h. ,balkew m. ,warburg a. ,hailu a. ,gebre-michael t.
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منبع
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parasites and vectors - 2015 - دوره : 8 - شماره : 1
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چکیده
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Background: blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. however,in ethiopia and other parts of east africa there are large remaining gaps in identifying the feeding habits of phlebotomus orientalis,the vector of leishmania donovani. the aim of the study was to determine the blood feeding patterns of p. orientalis in tahtay adiyabo district,northern ethiopia. methods: for bloodmeal analysis,sandflies were collected from three different villages of tahtay adiyabo district using cdc light traps,sticky traps,and pyrethrum spray catches. bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-pcr and reverse-line blotting (rlb) and enzyme linked immunosorbent assay (elisa) assays. results: most (637/641) of the females analyzed were p. orientalis. successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b pcr-rlb and elisa,respectively. bloodmeal analysis of p. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey,human,goat,sheep,dog,and camel. conclusion: results obtained from bloodmeal analyses demonstrate that the feeding preference of p. orientalis is mainly zoophilic,which could vary depending on the availability of hosts. © 2015 gebresilassie et al.; licensee biomed central.
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کلیدواژه
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Bloodmeal; Host preference; Phlebotomus orientalis; Tahtay Adiyabo; Visceral leishmaniasis
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آدرس
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department of zoological sciences,addis ababa university,addis ababa,ethiopia,department of biology,college of natural science,jigjiga university,jigjiga, Ethiopia, department of microbiology and molecular genetics,hebrew university,hadassah medical school,jerusalem, Israel, department of zoological sciences,addis ababa university,addis ababa, Ethiopia, department of biology,college of natural science,jigjiga university,jigjiga, Ethiopia, department of microbiology and molecular genetics,hebrew university,hadassah medical school,jerusalem, Israel, department of microbiology and molecular genetics,hebrew university,hadassah medical school,jerusalem, Israel, department of zoological sciences,addis ababa university,addis ababa, Ethiopia, aklilu lemma institute of pathobiology,addis ababa university,addis ababa, Ethiopia, department of microbiology and molecular genetics,hebrew university,hadassah medical school,jerusalem, Israel, department of microbiology,immunology and parasitology,college of health sciences,addis ababa university,addis ababa, Ethiopia, aklilu lemma institute of pathobiology,addis ababa university,addis ababa, Ethiopia
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Authors
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