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Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
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نویسنده
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marangi m. ,koehler a.v. ,zanzani s.a. ,manfredi m.t. ,brianti e. ,giangaspero a. ,gasser r.b.
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منبع
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parasites and vectors - 2015 - دوره : 8 - شماره : 1
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چکیده
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Background: cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. the aim of this study was to investigate the presence of cyclospora in captive non-human primates. methods: a total of 119 faecal samples from pan troglodytes,macaca sylvanus,cercopithecus cephus,erythrocebus patas,chlorocebus aethiops and macaca fascicularis from a wildlife animal rescue center as well as from macaca fascicularis from an experimental primate research center were tested for the presence of cyclospora by quantitative real-time pcr (qpcr) and single-strand conformation polymorphism (sscp) analysis. results: cyclospora was detected in three pan troglodytes (13.6%) and nine (9.3%) macaca fascicularis. conclusions: the present study represents the first record of cyclospora in captive primates in europe,suggesting the presence of cyclospora cayetanensis,which is transmissible to humans. © 2015 marangi et al.
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کلیدواژه
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Cyclospora; Mutation scanning-based sequencing; Non-human primates; qPCR; Zoonosis
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آدرس
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department of science of agriculture,food and environment,university of foggia,foggia,italy,faculty of veterinary and agricultural sciences,university of melbournevic, Australia, faculty of veterinary and agricultural sciences,university of melbournevic, Australia, department of animal pathology,hygiene and public health,university of milan,milan, Italy, department of animal pathology,hygiene and public health,university of milan,milan, Italy, department of veterinary sciences,university of messina,messina, Italy, department of science of agriculture,food and environment,university of foggia,foggia, Italy, faculty of veterinary and agricultural sciences,university of melbournevic, Australia
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Authors
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