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Phlebotomus papatasi SP15: MRNA expression variability and amino acid sequence polymorphisms of field populations
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نویسنده
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ramalho-ortigão m. ,coutinho-abreu i.v. ,balbino v.q. ,figueiredo c.a.s. ,mukbel r. ,dayem h. ,hanafi h.a. ,el-hossary s.s. ,fawaz e.e.-d.y. ,abo-shehada m. ,hoel d.f. ,stayback g. ,wadsworth m. ,shoue d.a. ,abrudan j. ,lobo n.f. ,mahon a.r. ,emrich s.j. ,kamhawi s. ,collins f.h. ,mcdowell m.a.
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منبع
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parasites and vectors - 2015 - دوره : 8 - شماره : 1
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چکیده
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Background: the phlebotomus papatasi salivary protein ppsp15 was shown to protect mice against leishmania major,suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-leishmania vaccines. methods: here,we investigated ppsp15 predicted amino acid sequence variability and mrna profile of p. papatasi field populations from the middle east. in addition,predicted mhc class ii t-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. results: the analysis of ppsp15 expression from field populations revealed significant intra- and interpopulation variation. in spite of the variability detected for p. papatasi populations,common epitopes for mhc class ii binding are still present and may potentially be used to boost the response against le. major infections. conclusions: conserved epitopes of ppsp15 could potentially be used in the development of a salivary gland antigen-based vaccine. © 2015 ramalho-ortigão et al.
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کلیدواژه
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Expression variability; Leishmaniasis; MHC class II epitopes; PpSP15; Saliva; Sand fly; Vaccine
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آدرس
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department of entomology,kansas state university,manhattan,ks 66506, United States, laboratory of malaria and vector research,niaid-nih,12735 twinbrook parkway,rockville,md 20852, United States, department of genetics,universidade federal de pernambuco,recife,pe, Brazil, jr.,department of genetics,universidade federal de pernambuco,recife,pe, Brazil, faculty of veterinary medicine,jordan university of science and technology,irbid,22110, Jordan, faculty of veterinary medicine,jordan university of science and technology,irbid,22110, Jordan, faculty of veterinary medicine,jordan university of science and technology,irbid,22110, Jordan, vector biology research program,u.s. naval medical research unit no. 3 (namru-3),cairo, Egypt, vector biology research program,u.s. naval medical research unit no. 3 (namru-3),cairo, Egypt, faculty of veterinary medicine,jordan university of science and technology,irbid,22110, Jordan, department of biostatistics,uniformed services university of the health sciences,bethesda,md 20814, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556, United States, department of pathology and laboratory medicine,children's hospital of philadelphia,philadelphia, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556, United States, department of biology,central michigan university,mount pleasant,detroit,mi 48859, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556,united states,department of computer science and engineering,university of notre dame,notre dame,in 46556, United States, laboratory of malaria and vector research,niaid-nih,12735 twinbrook parkway,rockville,md 20852, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556,united states,department of computer science and engineering,university of notre dame,notre dame,in 46556, United States, eck institute for global health,department of biological sciences,university of notre dame,notre dame,in 46556, United States
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Authors
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