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Genetic variability within and among Haemonchus contortus isolates from goats and sheep in China
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نویسنده
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yin f. ,gasser r.b. ,li f. ,bao m. ,huang w. ,zou f. ,zhao g. ,wang c. ,yang x. ,zhou y. ,zhao j. ,fang r. ,hu m.
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منبع
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parasites and vectors - 2013 - دوره : 6 - شماره : 1
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چکیده
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Background: haemonchus contortus (order strongylida) is a common parasitic nematode infecting small ruminants and causing significant economic losses worldwide. knowledge of genetic variation within and among h. contortus populations can provide a foundation for understanding transmission patterns,the spread of drug resistance alleles and might assist in the control of haemonchosis. methods. 152 h. contortus individual adult worms were collected from seven different geographical regions in china. the second internal transcribed spacer (its-2) of the nuclear ribosomal dna and mitochondrial nicotinamide dehydrogenase subunit 4 gene (nad4) were amplified by polymerase chain reaction (pcr) and sequenced directly. the sequence variations and population genetic diversities were determined. results: nucleotide sequence analyses revealed 18 genotypes (its-2) and 142 haplotypes (nad4) among the 152 worms,with nucleotide diversities of 2.6% and 0.027,respectively,consistent with previous reports from other countries,including australia,brazil,germany,italy,malaysia,sweden,the usa and yemen. population genetic analyses revealed that 92.4% of nucleotide variation was partitioned within populations; there was no genetic differentiation but a high gene flow among chinese populations; some degree of genetic differentiation was inferred between some specimens from china and those from other countries. conclusions: this is the first study of genetic variation within h. contortus in china. the results revealed high within-population variations,low genetic differentiation and high gene flow among different populations of h. contortus in china. the present results could have implications for studying the epidemiology and ecology of h. contortus in china. © 2013 yin et al.; licensee biomed central ltd.
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کلیدواژه
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China; Genetic variation; Haemonchus contortus; ITS-2; nad4
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آدرس
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state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, faculty of veterinary science,university of melbourne,parkville,vic 3010, Australia, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, college of animal sciences and veterinary medicine,liaoning medical college,jingzhou 121000, China, department of veterinary medicine,college of animal science and technology,guangxi university,nanning 530004, China, college of animal science and technology,yunnan agricultural university,kunming 650201, China, college of veterinary medicine,northwest aandf university,yangling 712100, China, college of animal science and veterinary medicine,heilongjiang bayi agricultural university,daqing 163319, China, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China, state key laboratory of agricultural microbiology,college of veterinary medicine,huazhong agricultural university,wuhan 430070, China
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Authors
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