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Isolation of a mitogenic agglutinin with relatively high thermostability from seeds of the variegated shell ginger
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نویسنده
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wong j.h. ,ng t.b. ,zhang k.y.b. ,sze s.c.w. ,wang h.x.
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منبع
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protein and peptide letters - 2010 - دوره : 17 - شماره : 1 - صفحه:38 -43
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چکیده
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An agglutinin with a molecular mass of 130 kda has been isolated from the seeds of alpinia zerumbet cv.'variegata'. the isolation procedure involved anion exchange chromatography on deae-cellulose,affinity chromatography on affi-gel blue gel,and gel filtration by fast protein liquid chromatography on superdex 75. the agglutinin exhibited hemagglutinating activity toward rabbit erythrocytes which could not be inhibited by simple sugars. it was composed of four identical 32-kda subunits with substantial n-terminal sequence similarity to chitinase and yieldin. the hemagglutinating activity of a. zerumbet agglutinin was stable up to 80°c and not affected by presence of a variety of salts. the agglutinin stimulated [methyl-3h]-thymidine uptake by mouse splenocytes. it did not exhibit antifungal activity. © 2010 bentham science publishers ltd.
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کلیدواژه
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Agglutinin; Alpinia zerumbet; Isolation; Mitogenic; Seeds
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آدرس
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school of biomedical sciences,faculty of medicine,the chinese,university of hong kong,shatin,new territories, China, school of biomedical sciences,faculty of medicine,the chinese,university of hong kong,shatin,new territories, China, school of chinese medicine,the university of hong kong,sassoon road, China, school of chinese medicine,the university of hong kong,sassoon road, China, state key laboratory for agribiotechnology and department of microbiology,china agricultural university, China
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Authors
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