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Annotation of Non-synonymous single polymorphisms in human liver proteome by mass spectrometry
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نویسنده
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chen m. ,yang b. ,ying w. ,he f. ,qian x.
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منبع
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protein and peptide letters - 2010 - دوره : 17 - شماره : 3 - صفحه:277 -286
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چکیده
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A novel strategy to annotate nssnp-peptides in human liver proteome based on lc-esi-ms/ms and peptide database search was proposed. totally 115 nssnp-peptides in human liver proteins were annotated using our method. among them,42 peptides were found to be amino acid mutation,73 peptides were wild type,5 peptides were interpreted with both mutation and wild type. the function of nssnp-peptide was predicted using sift algorithm,and 2 nssnps were predicted to be damaged for protein function. the results here show that the strategy is very effective for annotation of nssnp at peptide level. © 2010 bentham science publishers ltd.
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کلیدواژه
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Liquid chromatography-tandem mass spectrometry; Liver proteins; nsSNP; Peptide database; SIFT
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آدرس
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state key laboratory of proteomics,beijing proteome research center,beijing institute of radiation medicine,beijing 100850,china,key laboratory of stress medicine,tianjing institute of hygiene and environmental medicine, China, state key laboratory of proteomics,beijing proteome research center,beijing institute of radiation medicine, China, state key laboratory of proteomics,beijing proteome research center,beijing institute of radiation medicine, China, state key laboratory of proteomics,beijing proteome research center,beijing institute of radiation medicine, China, state key laboratory of proteomics,beijing proteome research center,beijing institute of radiation medicine, China
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Authors
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