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The protective effects of osmolytes on yeast alcohol dehydrogenase conformational stability and aggregation
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نویسنده
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han h.-y. ,yao z.-g. ,gong c.-l. ,xu w.-a.
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منبع
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protein and peptide letters - 2010 - دوره : 17 - شماره : 8 - صفحه:1058 -1066
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چکیده
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The protective effects of four osmolytes (trehalose,dimethysulfoxide,glycine and proline) on the conformational stability and aggregation of guanidine-denatured yeast alcohol dehydrogenase (yadh) have been investigated in this paper. the results show that the four osmolytes protect yadh against unfolding and inactivation by reducing ki (inactivation rate constants),increasing δδgi (transition free energy changes at 25°c),increasing cm (value for the midpoint of denaturation) and decreasing its ans-binding fluorescence intensity. furthermore,these osmolytes can prevent yadh aggregation in a concentration-dependent manner during yadh refolding. © 2010 bentham science publishers ltd.
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کلیدواژه
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Aggregation; Conformational stability; Osmolytes; Transition free energy; Unfolding; Yeast alcohol dehydrogenase
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آدرس
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department of biology,medical college of soochow university,jiangsu, China, department of life science,binzhou university,shandong, China, department of biology,medical college of soochow university,jiangsu, China, department of biology,medical college of soochow university,jiangsu, China
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Authors
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