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Expression,purification,crystallization and preliminary x-ray crystallographic analysis of the peptidoglycan binding region of the ser/thr kinase PrkC from staphylococcus aureus
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نویسنده
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ruggiero a. ,squeglia f. ,izzo v. ,silipo a. ,vitagliano l. ,molinaro a. ,berisio r.
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منبع
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protein and peptide letters - 2010 - دوره : 17 - شماره : 10 - صفحه:1296 -1299
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چکیده
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Prkc is an important ser/thr membrane kinase of staphylococcus aureus able to bind peptidoglycans through extra-cellular domains,denominated as pasta. upon peptidoglycan binding,prkc is activated and stimulates bacterial growth and revival from latency. the entire extra-cellular region of prkc (residues 378-664),containing three predicted pasta domains and an extra-domain of unknown function,has been successfully crystallized using vapor-diffusion methods. the structure has been solved by multi wave length anomalous dispersion and refinement is in progress. © 2010 bentham science publishers ltd.
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کلیدواژه
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Cell wall; Crystal; Latency; X-ray
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آدرس
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institute of biostructures and bioimaging,cnr,napoli,italy,university of naples federico ii, Italy, institute of biostructures and bioimaging,cnr,napoli,italy,university of naples federico ii, Italy, university of naples federico ii, Italy, institute of biostructures and bioimaging,cnr, Italy, institute of biostructures and bioimaging,cnr, Italy, university of naples federico ii, Italy, institute of biostructures and bioimaging,cnr, Italy
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Authors
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