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Quantitative effects of magnesium chloride stress on aggregation of sup35p in [psī] yeast cells
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نویسنده
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song y. ,lan w. ,wu x. ,he j. ,li h. ,ben s. ,song y.
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منبع
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protein and peptide letters - 2010 - دوره : 17 - شماره : 12 - صفحه:1489 -1494
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چکیده
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[psi+] phenotype can be transiently induced when magnesium chloride (mgcl2) was the selective pressure in sup35 repeat-expansion mutant [psī] yeast strains. we further investigated [psi+] phenotype change under different mgcl2 conditions with native sup35p and quantified the sup35p status changes with fluorescence recovery after pho-tobleaching (frap) and semi-denaturing detergent-agarose gel electrophoresis (sdd-age) analysis. it was found that the [psi+] phenotype presented a dose-dependent relationship with the concentrations of mgcl2. furthermore,sup35p aggregated in mgcl2 treated cells but did not form large aggregates as it does in [psi+] cells,and the size of sup35p aggregates showed a time-dependent relationship with the mgcl2 application. the aggregation of sup35p strictly depended on the presence of mgcl2 stress in our strains. © 2010 bentham science publishers ltd.
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کلیدواژه
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[PSI+]; Magnesium chloride; Prion; Quantitative effects; Stress.; Sup35p
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آدرس
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school of life science,liaoning university, China, school of life science,liaoning university, China, school of life science,liaoning university, China, school of life science,liaoning university,shenyang 110036,china,province key laboratory of animal resource and epidemic disease prevention, China, school of life science,liaoning university,shenyang 110036,china,province key laboratory of animal resource and epidemic disease prevention, China, school of life science,liaoning university, China, school of life science,liaoning university,shenyang 110036,china,province key laboratory of animal resource and epidemic disease prevention, China
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Authors
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