An antifungal peptide with antiproliferative activity toward tumor Cells from red kidney beans

A 7.3-kda antifungal peptide was purified from dried red kidney beans. the purification procedure entailed ion exchange chromatography on deae-cellulose,affinity chromatography on affi-gel blue gel,ion exchange chromatography on cm-cellulose,followed by fast protein liquid chromatography-gel filtration on superdex 75. the peptide was unadsorbed on deae-cellulose but adsorbed on affi-gel blue gel and cm-cellulose. it exhibited a molecular mass of 7.3 kda in gel filtration and also in sds-polyacrylamide gel electrophoresis,indicating that it is a single-chained protein. the n-terminal sequence of the peptide was dgvcfgglangdrt. the peptide exerted an antifungal action on fusarium oxysporum with an ic50 of 3.8±0.4 μm (mean±sd,n=3). it also inhibited mycelial growth in mycosphaerella arachidicola. it suppressed growth of lymphoma mbl2 cells and leukemia l1210 cells with an ic50 of 5.2±0.4 μm and 7.6±0.6 μm,respectively. hiv-1 reverse transcriptase was inhibited with an ic50 of 40±3.2 μm. however,no activity was demonstrated toward other viral enzymes. © 2011 bentham science publishers ltd.