Impact of microencapsulated peptidase (aspergillus oryzae) on cheddar cheese proteolysis and its biologically active peptide profile

We investigated the delivery of calcium-alginate encapsulated peptidase (flavourzyme®,aspergillus oryzae) on proteolysis of cheddar cheese. physical and chemical characteristics such as moisture,ph and fat content were measured,and no differences were found between control and experimental cheese at day 0. sds-page analysis clearly showed that proteolysis of β and k casein was significantly accelerated after three months of maturity in the experimental cheese. a large number of low molecular weight peptides were found in the water soluble fraction of the experimental cheeses and some of these peptides were new. n-terminal amino acid sequence analysis identified these as p 1,leu-thu- glu; p 3,asp-val-pro-ser-glu and relatively abundant stable peptides p 2,p 4,arg-pro-lys-his-pro-ile; p 5,arg-pro-lys- his-pro-ile-lys and p 6. these peptides were mainly originated from βs1-cn and β-cn. four of the identified peptides (p 1,p 2,p 3 and p 4) are known to be biologically active and p 1 and p 3 were only present in experimental cheese suggesting that experimental cheese has improved health benefits. © 2011 bentham science publishers ltd.