Analysis of rate-limiting long-range contacts in the folding rate of three-state and two-state proteins

In the past decade,when compared to models describing the folding rates of two-state proteins,models describing the folding mechanism of three-state proteins remain quite limited due to the complexity present in the folding mechanism and lack in their experimental data. in the present work,rate-limiting long-range contacts were classified into various bins based on sequence separation distance between the contacting residues and the role of these bins were analyzed for their importance in a data set of 35 three-state proteins. predicting the folding rates of these proteins have been carried out by relating experimental folding rates and long-range contacts obtained from various sequence separation bins. for comparison,using the present model,prediction of the folding rates of 45 two-state proteins also resulted with good accuracy. our method shows that long-range contacts observed in the final 3-d structure of proteins at various sequence separation bins are found to be an important descriptor in explaining the folding rates of three-state proteins and suggest that formation of contacts between residues present at these sequence separation distance may be a crucial factor in deciding structure formation and folding rates of these proteins. the aim of our present work is not to construct a new descriptor for the folding rates of three-state proteins,nor is to provide improved means of folding-rate prediction for these proteins. we tend to elucidate that how long-range contacts play a crucial role in the folding mechanism of three state proteins belonging to three major structural classes and implication of these observations due to rate-limiting long-range contacts has been discussed in the light of other experimental studies of protein folding. © 2011 bentham science publishers.