Prostaglandin E2 binding peptide: A potential modulatory agent which acts through suppressing NF-kappaB signaling in RA

In an earlier study,we found pbp inhibited the progress of adjuvant-induced arthritis (aa). this study was aimed at evaluating the inhibitory effects of pbp in terms of nf-κb activation by using immunohistochemical and immunofluorescent technique in vitro and in vivo. il-1β and tnf-α in serum were detected by method of elisa. immunofluorescent results showed that pbp inhibited nf-κb p65 translocation into nucleus. in vivo imaging showed that treatment with pbp decreased the enzyme labeling signal of nf-κb p65. immunohistochemical staining revealed that pbp suppressed production of nf-κb p65 subunit in the joints and attenuated the productions of il-1β and tnf-α in serum from aa. moreover,nf- κb p65 nucleus translocation was prevented by simultaneous incubation with pbp and pge2 was decreased by pbp through a feedback cycle. we report the first confirmation of the mimotope of pge2 receptor ep4 modulatory action. © 2012 bentham science publishers.