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D-serine-dehydratase from saccaromyces cerevisiae: A pyridoxal 5'-phosphate-dependent enzyme for advanced biotech applications
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نویسنده
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staiano m. ,strianese m. ,varriale a. ,giovanni s.d. ,mase d.s.d. ,ç'angelo v. ,ruggiero g. ,labella t. ,pellecchia c. ,d'auria s.
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منبع
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protein and peptide letters - 2012 - دوره : 19 - شماره : 6 - صفحه:592 -595
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چکیده
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The saccaromices cerevisiae d-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. it catalyses the conversion of d-serine into pyruvate and ammonia with the k(m) and k(cat) values of 0.39 mm and 13.1 s(-1) respectively. in this work,a new methodology for monitoring d-serine is presented. our results show that this enzyme could be successfully used as a biological probe for detection of d-serine via fluorescence spectroscopy. © 2012 bentham science publishers.
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کلیدواژه
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Absorption; Biosensors; D-Serine dehydratase; Fluorescence
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آدرس
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laboratory for molecular sensing,ibp-cnr,via pietro castellino,111 80131 naples,italy, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino,111 80131 naples,italy, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy, laboratory for molecular sensing,ibp-cnr,via pietro castellino, Italy
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Authors
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