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Comparison of the neutrophil proteome in trauma patients and normal controls
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نویسنده
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teles l.m.b. ,aquino e.n. ,neves a.c.d. ,garcia c.h.s. ,roepstorff p. ,fontes b. ,castro m.s. ,fontes w.
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منبع
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protein and peptide letters - 2012 - دوره : 19 - شماره : 6 - صفحه:663 -672
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چکیده
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Background: neutrophils have an impressive array of microbicidal weapons,and in the presence of a pathogen,progress from a quiescent state in the bloodstream to a completely activated state. failure to regulate this activation,for example,when the blood is flooded with cytokines after severe trauma,causes inappropriate neutrophil activation that paradoxically,is associated with tissue and organ damage. acidic proteomic maps of quiescent human neutrophils were analyzed and compared to those of activated neutrophils from severe trauma patients. the analysis revealed 114 spots whose measured volumes differed between activated and quiescent neutrophils,with 27 upregulated and 87 downregulated in trauma conditions. among the identified proteins,grancalcin,s100-a9 and cacnb2 reinforce observed correlations between motility and ion flux,anxa3,snap,fgd1 and zfyve19 are involved in vesicular transport and exocytosis,and gstp1,hspa1 hspa1l,maob,uch-l5,and ppa1 presented evidence that activated neutrophils may have diminished protection against oxidative damage and are prone to apoptosis. these are discussed,along with proteins involved in cytoskeleton reorganization,reactive oxygen species production,and ion flux. proteins such as zfyve19,maob and albumin- like protein were described for the first time in the neutrophil. in this work we achieved the identification of several proteins potentially involved in inflammatory signaling after trauma,as well as proteins described for the first time in neutrophils. © 2012 bentham science publishers.
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کلیدواژه
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Inflammation; Neutrophils; Proteome; Systemic inflammatory response syndrome; Trauma
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آدرس
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laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília, Brazil, laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília, Brazil, laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília, Brazil, laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília,df,brazil,department of biochemistry and molecular biology,university of southern denmark, Denmark, department of biochemistry and molecular biology,university of southern denmark, Denmark, laboratory of medical investigation (lim-62),3rd division of clinical surgery, Brazil, laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília,df,brazil,laboratory of toxinology,physiological sciences department, Brazil, laboratory of biochemistry and protein chemistry,cell biology department,university of brasília,brasília, Brazil
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Authors
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