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Site-directed mutagenesis study of the Ile140 in conserved hydrophobic core of Bcl-xL
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نویسنده
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zhang x. ,tan y. ,zhao r. ,chu b. ,tan c. ,jiang y.
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منبع
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protein and peptide letters - 2012 - دوره : 19 - شماره : 9 - صفحه:991 -996
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چکیده
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The hydrophobic core in bcl-xl composed of trp137,ile140,trp181,ile182,trp188 and phe191 is highly conserved and essential for protein folding,protein stability and binding affinity with bh3-peptide. 9 mutants of ile140 residue were constructed and characterized in order to get better understanding of the effect of the hydrophobic core. binding assay demonstrated that binding affinities between 4 charged mutants and bh3-peptide were significantly weakened or lost,suggesting that the integrity of the hydrophobic core has close relationship with binding. the cd spectroscopy results indicated that disruption of the hydrophobic core may affect local conformation within the protein and result in intrinsic inactivity. further chemical-induced protein folding results on these 4 mutants revealed that the conserved hydrophobic core is also important for the protein stability. © 2012 bentham science publishers.
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کلیدواژه
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Bad-BH3; Bcl-xL; Binding; Hydrophobic core; Site-directed mutagenesis; Stability
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آدرس
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department of chemistry,tsinghua university,beijing 100084,china,ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university, China, ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university, China, department of chemistry,tsinghua university,beijing 100084,china,ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university, China, department of chemistry,tsinghua university,beijing 100084,china,ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university, China, department of chemistry,tsinghua university,beijing 100084,china,ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university, China, ministry-province jointly constructed base for state key lab,graduate school at shenzhen,tsinghua university,shenzhen 518055,china,department of pharmacology and pharmaceutical sciences,school of medicine,tsinghua university, China
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Authors
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