Characterization and decolorization applicability of xerogel matrix immobilized manganese peroxidase produced from trametes versicolor IBL-04

A novel manganese peroxidase (mnp) isolated from solid state culture of trametes versicolor ibl-04 was immobilized using xerogel matrix composed of trimethoxysilane (tmos) and propyltetramethoxysilane (ptms). ftir spectroscopy confirmed the successful entrapment of mnp into the xerogel matrix. an immobilization efficiency of 92.2% was achieved with a purified active fraction containing 2 mg/ml mnp. after 24 h incubation at varying ph and temperatures,the immobilized mnp retained 82 and 75% activity at ph 4 and 80 °c,respectively. xerogel matrix immobilization enhanced the catalytic efficiency of entrapped mnp. metal ions including cu2+,mn2+ and fe2+ stimulated enzyme activity while cysteine,edta and ag+ inhibited the activity. mnp preserved 82% of its initial activity during oxidation of mnso4 in 10 consecutive cycles,demonstrating the reusability of xerogel entrapped mnp. the immobilized mnp could be stored for up to 75 days at 4 °c without significant activity loss. to explore the industrial applicability of mnp,the immobilized mnp was tested for decolorization of textile industry effluent in a packed bed reactor system (pbrs). after five consecutive cycles,98.8% decolorization of effluent was achieved within 5 h. the kinetic properties,storage stability and reusability of entrapped mnp from t. versocolor ibl-04 reflect its prospects as biocatalyst for bioremediation and other industrial applications. © 2013 bentham science publishers.