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Aqueous microwave-assisted solid-phase peptide synthesis using Fmoc strategy. II. Racemization studies and water based synthesis of cysteine-containing peptides
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نویسنده
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hojo k. ,shinozaki n. ,hara a. ,onishi m. ,fukumori y. ,ichikawa h.
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منبع
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protein and peptide letters - 2013 - دوره : 20 - شماره : 10 - صفحه:1122 -1128
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چکیده
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We have developed a microwave (mw)-assisted peptide synthesis using fmoc-amino acid nanoparticles in water previously. it is an organic solvent-free,environmentally friendly method for peptide synthesis. in this study,we have investigated the racemization of cysteine during an aqueous based coupling reaction with mw irradiation. under our mw-assisted protocol using wsci and dmtmm,the coupling reaction can be performed with low levels of racemization of cysteine. we also demonstrated the synthesis of the nonapeptide oxytocin analogue,cys(acm)-tyr-ile-gln-asn-cys(acm)-pro-leu-gly-nh2 using our water based mw-assisted protocol with fmoc-amino acid nanoparticles. © 2013 bentham science publishers.
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کلیدواژه
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Cysteine; Microwave-assisted synthesis; Nanoparticles; Racemization solid-phase peptide synthesis; Synthesis in water
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آدرس
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faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan, faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan, faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan, faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan, faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan, faculty of pharmaceutical sciences,cooperative research center of life sciences,kobe gakuin university,1-1-3 minatojima,chuo-ku, Japan
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Authors
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