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CPP-mediated protein delivery in a noncovalent form: Proof-of-concept for percutaneous and intranasal delivery
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نویسنده
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wang z. ,chen y. ,liu e. ,gong j. ,shin m.c. ,huang y.
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منبع
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protein and peptide letters - 2014 - دوره : 21 - شماره : 11 - صفحه:1129 -1136
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چکیده
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Macromolecular drugs (e.g.,proteins and nucleic acids) are highly environmentally liable and unstable,and their administration is strictly limited to injection. moreover,a vast majority of macromolecules are cell membraneimpermeable,and it is a critical issue to enhance the cellular uptake efficiency for improving the treatment outcomes. cell-penetrating peptide (cpp)-assisted strategy is promising for effective macromolecular delivery. as a case in point,cpp-mediated protein delivery has been considered as a revolutionary breakthrough. with aid of cpp,virtually all proteins can become cell-permeable. generally,cpp-protein delivery works in a covalent delivery pattern,by which cpp and its cargo are linked via covalent bond. recently,noncovalent delivery has also attracted attention for its potential application for protein delivery. in the presented work,the noncovalent pattern was demonstrated for its feasibility in percutaneous and nose-to-brain delivery with tat/gfp as model drug,in comparison with the covalent method. noncovalent cpp/protein delivery and its noninvasive application may provide a facile method for protein therapy. © 2014 bentham science publishers.
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کلیدواژه
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Cell-penetrating peptide; Noncovalent; Nose-to-brain delivery; Percutaneous delivery; Protein delivery; TAT
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آدرس
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shanghai institute of materia medica,chinese academy of sciences,501 haike rd, China, shanghai institute of materia medica,chinese academy of sciences,501 haike rd, China, shanghai institute of materia medica,chinese academy of sciences,501 haike rd,shanghai,china,state key laboratory of chemical engineering,school of chemical engineering and technology,tianjin university,92 weijin rd, China, state key laboratory of chemical engineering,school of chemical engineering and technology,tianjin university,92 weijin rd, China, university of michigan college of pharmacy,428 church st.,ann arbor, United States, shanghai institute of materia medica,chinese academy of sciences,501 haike rd, China
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Authors
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