Solid lipid nanoparticles loaded with fluorescent-labelled cyclosporine A: Anti-inflammatory activity in vitro

Fmoc-isocyclosporine a,a fluorescent labeled cyclosporine a,was encapsulated in solid lipid nanoparticles (sln) prepared by the coacervation technique,and its anti-inflammatory activity was evaluated. the anti-inflammatory activity of the fluorescent labelled molecule,measured as inhibition of tnf-α secretion,is similar to the native one. sln were compared to commercial formulations,through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. drug-loaded sln inhibit tnf-α secretion in a lower extent than commercial formulations,probably due to a lower uptake by the cells,but the increase of il-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity. © 2014 bentham science publishers.