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A novel strategy for preparing glycopeptide molecular probe using fluorous technology
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نویسنده
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liu z. ,rong p. ,gu x. ,yu l. ,chen g. ,chen y.-l. ,zhang s. ,shen j. ,zeng w.
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منبع
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protein and peptide letters - 2014 - دوره : 21 - شماره : 10 - صفحه:1011 -1016
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چکیده
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A novel and convenient strategy for iodine labeled glycopeptide molecular probe and purification was developed. the fluorine rich bi-functional coupling agent,4-tris(2-perfluorohexylethyl)stannylbenzoate succinimidyl ester,was successfully synthesized via 5 steps starting from the fluorous grignard reagent. it was purified by a simple and fast isolation using perfluorinated hexanes (fc-72). the cold iodine labeled yield for the coupling agent was as high as 92% within 15 min. the iodine-labeled product was only in organic fractions as we expected. it was shown that there was only one major peak in organic fractions according to hplc. finally,the iodine-labeled coupling agent was applied to label glycopeptide and afforded a high yield of 87% within 30 min. © 2014 bentham science publishers.
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کلیدواژه
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Fluorous technology; Glycopeptide; Iodination; Molecular probe
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آدرس
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school of pharmaceutical sciences,central south university,172 tongzipo road, China, school of pharmaceutical sciences,central south university,172 tongzipo road, China, shanghai institute of materia medica,chinese academy of sciences,555 zuchongzhi road, China, school of pharmaceutical sciences,central south university,172 tongzipo road, China, shanghai institute of materia medica,chinese academy of sciences,555 zuchongzhi road, China, school of pharmacy,china pharmaceutical university,24 tongjiaxiang, China, department of otolaryngology,xiangya hospital,central south university, China, school of pharmacy,china pharmaceutical university,24 tongjiaxiang, China, school of pharmaceutical sciences,central south university,172 tongzipo road, China
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Authors
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