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The expression of relaxin-3 in adipose tissue and its effects on adipogenesis
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نویسنده
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yamamoto h. ,shimokawa h. ,haga t. ,fukui y. ,iguchi k. ,unno k. ,hoshino m. ,takeda a.
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منبع
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protein and peptide letters - 2014 - دوره : 21 - شماره : 6 - صفحه:517 -522
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چکیده
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Energy homeostasis is regulated by endocrine factors. the concentration of relaxin-3 in serum is related to body mass index. however,relaxin-3 is found only in the brain and testis. in this study,we examined the expression of relaxin-3 in adipose tissue and its effects on adipogenesis. the expression of relaxin-3 was determined using rt-pcr,a relaxin-3 c-peptide-specific radioimmunoassay,specifically in the stromal-vascular fraction (svf) cells rather than adipocytes. the release of c-peptide was regulated by glucose concentration in the svf cells. however,the differentiated adipocytes did not express relaxin-3. in glucose perfusion experiments,c-peptide was released in response to high glucose concentrations in the mesenteric perfusate,opposite to insulin release. additionally,gpcr135 mrna was expressed in adipocytes. relaxin-3 increased triglycerides in adipocytes and decreased lipase activity. the present study showed that relaxin-3 is secreted from svf cells and that it regulates lipid accumulation in adipocytes. © 2014 bentham science publishers.
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کلیدواژه
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Adipocyte; GPCR135; Lipid accumulation; Mesentery adipose tissue; Relaxin-3
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آدرس
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laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan, laboratory of bioorganic chemistry,school of pharmaceutical sciences,university of shizuoka,52-1 yada, Japan
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Authors
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