Comparative protein profiling of intraphagosomal expressed proteins of Mycobacterium bovis BCG

Bcg,the only available vaccine against tuberculosis affords a variable protection which wanes with time. in this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of m.bovis bcg. eight proteins which showed increased expression during the intraphagosomal growth were identified by maldi-tof/ms. these were - a precursor of alanine and proline-rich secreted protein apa,isoforms of malate dehydrogenase,large subunit alpha (alpha-etf) of electron transfer flavoprotein,immunogenic protein mpb64 precursor,upf0036 protein,and two proteins with unknown function. based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival,adaptation and/or immunoprotective effect of bcg. further,these proteins might also be used as gene expression markers for endosome trafficking events of bcg. © 2015 bentham science publishers.