Determination of flavonoids and anthocyanins in nitraria tangutorum by high performance liquid chromatography coupled with tandem mas s spectrometry

Using high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (hplc-dad-msn) method,qualitative and quantitative analysis of flavonoids of stems,leaves,fruits and seeds,and anthocyanidin of fresh fruits in nitraria tangutorum were performed. a total of 14 flavonoid components were identified from the seeds of n. tangutorum including three quercetin derivatives,three kaempferol derivatives,and eight isorhamnetin derivatives. a total of 12,10,and 7 flavonoid components were identified from leaves,stems,and fruits of n. tangutorum,respectively; all were present in seeds also. the total content of flavonoids in leaves was the highest,up to 42.43 mg/gdry weight. a total of 12 anthocyanidin components were identified from the fresh fruits of n. tangutorum,belonging to five anthocyanidin. the total content of anthocyanidin in fresh fruits was up to 45.83 mg/100 gfresh weight,of which the acylated anthocyanidin accounted for 65.7%. the hplc-dad-msn method can be operated easily,rapidly,and accurately,and is feasible for qualitative and quantitative analysis of flavone glycosides in n. tangutorum. © 2016 bentham science publishers.