Cloning and transformation of Eehkt14 gene from elytrigia elongate

The eehkt1;4 gene was firstly cloned from elytrigia elongata by rt-pcr technique with 1977 bp full-length cdna encoding 1722 bp open reading frame (orf) and 573 amino acids. the pcr fragment of eehkt1;4 gene was inserted into the binary vector pbi121 and got the resulted expression vector,which named pbi121-35s-eehkt1;4-nos. the vector was further transformed into the agrobacterium eha105,and then eehkt1;4 gene was transferred into tobacco by the agrobaterium- mediated genetic transformation method. the results showed that the target gene was inserted into the genomes of tobacco and expressed. therefore,the transgenic tobacco (t0) plants overexpressing eehkt1;4 gene were successfully obtained in this study. and eehkt1;4 reduces na+ concentration in the leaves of t0 plants,thereby plays a central role in protecting plant leaves from salinity stress. © 2016 bentham science publishers.