Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesis

In this work,we report the synthesis of a peptide analogue of the kttks,termed ac-wahx-kttks and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. the peptide was obtained with purity higher than 97.5%. in the cytotoxicity assay,we observed non-toxic effects for ac-wahx-kttks at concentrations below 600 ôm for hacat and 500 ôm for hepg2 cells,respectively. after 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. in conclusion,the ac-wahx-kttks peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging. © 2016 bentham science publishers.