Polyacrylamide gel-entrapped fungal manganese peroxidase from ganoderma lucidum IBL-05 with enhanced catalytic,stability,and reusability characteristics

In the present study,polyacrylamide gel (pag) was utilized as bolster material for the immobilization of in-house extracted and partially purified manganese peroxidase (mnp) through an entrapment technique yielding significant mnp immobilization (87.3±3.3 %) and remarkable stability of the enzyme (37.2±2.4 %) after a storage period of two months at 4°c. the immobilization also increased the optimal temperature by 10 °c and provided an alkaline shift of the ph optimum. moreover,a significant enhancement in the thermo-stability was observed. after an incubation period of 72 h at 50°c,the pag-entrapped-mnp still exhibited 41.2 % of the initial activity,whereas the free enzyme was completely inactive. furthermore,pag-entrapped-mnp showed an excellent recycling efficiency and retained more than 50% of its initial activity after five consecutive reaction cycles. in conclusion,owing to the economic feasibility,carrier-supported mnp may be a promising candidate for various applications in different industrial sectors. © 2016 bentham science publishers.